Analysis of cyclic nucleotide phosphodiesterase by isoelectric focusing coupled to a specific activity stain

The procedure described allowed a convenient analysis of cyclic nucleotide phosphodiesterase. The different phosphodiesterase forms present in a crude cytosolic preparation from rat heart were separated by isoelectric focusing on a polyacrylamide gel plate. Phosphodiesterase activity bands were rendered evident by a specific staining method. They were then characterized by means of their substrate specificity and their sensitivity to selective phosphodiesterase inhibitors. The correspondence between the stain bands and the previously described activity peaks, obtained by a preparative technique and detected by radioisotopic enzyme assay, was also investigated.     

Comparative Study of Fatty Acids Profile in Eleven Wild Mushrooms of Boletacea and Russulaceae Families

Eleven species of wild mushrooms which belong to Boletaceae and Russulaceae families were examined by gas chromatography (GC) and gas chromatography–mass spectrometry (GC/MS) analysis for the presence of fatty acids. As far as we know, the fatty acid profiles of B. purpureus and B. rhodoxanthus were described for the first time. Twenty‐six fatty acids were determined. Linoleic (19.5 – 72%), oleic (0.11 – 64%), palmitic (5.9 – 22%) and stearic acids (0.81 – 57%) were present in the highest contents. In all samples, unsaturated fatty acids dominate. Agglomerative hierarchical clustering was used to display the correlation between the fatty acids and their relationships with the mushroom species. Based on the fatty acids profile in the samples, the mushrooms can be divided into two families: Boletaceae and Russulaceae families, using cluster analysis.     

Cloning and characterization of KM190, a specific satellite DNA family of Drosophila kitumensis and D. microlabis

The nucleotide sequences of nine clones, pKA191/l-4 from Drosophila kitumensis and pMR.190/1–5 from D. microlabis, were determined. They represent a tandemly arranged and highly repetitive satellite DNA family, KM190, which is specific for the two species.     

On the electron delocalization in cyclopropenylidenes - An experimental charge-density approach

The extent and nature of cyclic electron delocalization in free and coordinated cyclopropenylidene carbenes has been analyzed by combined experimental and theoretical charge-density studies. The significant asymmetry of the C-C bond lengths in substituted cyclopropenylidene carbenes was identified as cooperative effect which depends on contributions of both σ- and π-bonding. We show that analyses of (i) the topology of the Laplacian of the electron density distribution and (ii) the out-of-plane atomic quadrupole moments - the charge-density analogues of p_π occupation - allow to distinguish between the influence of σ- and π-electrons on cyclic electron delocalization. These studies hint for pronounced electron localization in the carbene lone pair region which dominates the electronic structure of free cyclopropenylidene carbenes and hinders the establishment of true aromaticity. We further investigated the electron donating/withdrawing ability of cyclopropenylidene ligands relative to N-heterocyclic carbenes. The experimental benchmark systems LCr(CO)₅ (L = 2,3-diphenylcyclopropenylidene and 1,2-dimethylimidazol-2-ylidene) show that the cyclopropenylidene ligand clearly displays the higher π-acceptor capability relative to N-heterocyclic carbenes.     

Flood Risk Analysis Based on Information Diffusion Theory

Due to the fact that the flood data series of small drainage basins is relatively short, available data are often not sufficient for flood risk analysis. This presents the problem of risk analysis using very small data samples. One method that can be applied is to regard the available small samples as fuzzy information and optimize them using information diffusion technology to yield analytical results with greater reliability. In this article a risk analysis method based on information diffusion theory is applied to create a new flood risk analysis model. Application of the model is illustrated taking the Jinhuajiang and Qujiang drainage basins as examples. This is a new attempt at applying information diffusion theory in flood risk analysis. Computations based on this analytical flood risk model can yield an estimated flood damage value that is relatively accurate. This study indicates that the aforementioned model exhibits fairly stable analytical results, even when using a small set of sample data. The results also indicate that information diffusion technology is highly capable of extracting useful information and therefore improves system recognition accuracy. This method can be easily applied and the analytical results produced are easy to understand. Results are accurate enough to act as a guide in disaster situations.     

Repetitive changes in Early Pliocene vegetation revealed by high-resolution pollen analysis: revised cyclostratigraphy of southwestern Romania

A high-resolution pollen analysis has been carried out on the Lupoaia section (SW Romania) in order to check whether the repetitive clay–lignite alternations correspond to cyclic changes in climate. Increases in altitudinal tree pollen content appear to have been caused by drops in temperature, while developments of thermophilous elements correspond to rises in temperature, still under humid conditions. Such repeated changes in vegetation, on the whole consistent with the clay–lignite alternations, have been forced by cycles in eccentricity. On the basis of a comparison between the Lupoaia pollen record and (1) European climatostratigraphy (based on reference pollen diagrams documenting global changes), and (2) global climatic curves (eccentricity, δ¹⁸O), the age of the section has been reconsidered. The Lupoaia section (i.e. from lignite IV to lignite XIII) starts just before the C3n.3n Chron and probably ends just before the C3n.1n Chron. The section represents a time span of about 600 kyr, i.e. from about 4.90 Ma to about 4.30 Ma.     

Historical and recent evidence for close relationships among Rickettsia parkeri,R. conorii,R. africae,and R. sibirica: Implications for rickettsial taxonomy

Rickettsia parkeri, a member of the spotted fever group rickettsias, was first described in 1939 and was thought to be non‐pathogenic until recently, when it was found to cause a spotted fever‐like illness in humans and areas of necrosis (eschars) at the sites of tick bites. Accordingly, there is currently much interest in this emerging pathogen. In this study, all published articles concerning R. parkeri were reviewed and analyzed for evidence of relatedness among this agent and other spotted fever group (SFG) rickettsiae which also produce similar clinical syndromes and/or eschars, including R. conorii, R. africae, and R. sibirica. A synthesis of the historical (antigenic) and recent (molecular) data supporting a phylogenetic sub‐grouping of these SFG organisms is presented and comments are offered about the taxonomy of rickettsial organisms in general, and R. parkeri in particular.